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COMPARATIVE TRANSCRIPT LEVELS OF SUGAR METABOLISM GENES BETWEEN THE CANARY MELON AND A VIETNAMESE NON-SWEET MELON CULTIVAR

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Two different melon cultivars, Canary and Vietnamese non-sweetmelons, are used to compare the fruit’s sweetness levels. The resultsindicate that the Canary melon is much sweeter than the non-sweetmelon. The transcript levels of the sugar metabolism genes, includingCucumis melo ACID INVERTASE 2 (CmAIN2) and SUCROSESYNTHASE 1 (CmSUS1), are examined in two fruit tissues. PCR usingcDNA and the electrophoresis assays indicate that the CmAIN2 andCmSUS1 primer sets are specific, and only one band of PCR product isobtained from all tested samples. The quantitative reverse transcriptionPCR (RT-qPCR) assay is applied to compare the transcript levels ofthe CmAIN2 and CmSUS1 genes in fruit tissues of the Canary andthe Vietnamese non-sweet melons. Consistent with the sweetnesslevels, the CmAIN2 and CmSUS1 transcript levels are higher in theCanary melon than those in the non-sweet melon. These resultsimply that the local sugar metabolism in the fruits may also play anessential role in determining fruit sweetness. In addition, practically,the transcript levels of theCmAIN2 and CmSUS1 genes can beaccessed and used to predict the sweetness of melon fruits early.

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Ketersediaan

UIB00007610

Tersedia

Informasi Detail

Judul Seri

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No. Panggil

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Penerbit

Malang : Faculty of Agriculture Universitas Brawijaya., 2024

Deskripsi Fisik

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Bahasa

English

ISBN/ISSN

0126-0537

Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

Vol 46, No 1

Subjek

Agribisnis

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

Lampiran Berkas

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